Fluorescence of Glycogen Phosphorylase b

Fluorescence of glycogen phosphorylase b. Structural transitions and energy transfer.

The fluorescence properties of glycogen phosphorylase b and its apoenzyme are described. At neutral pH the enzyme has two fluorescence bands: one caused by the protein moiety (maximum at 335 nm, quantum yield 0.12) and the other associated with the cofactor, pyridoxal 5’-phosphate (maximum at 535 nm, quantum yield 0.012). Pyridoxal5’-phosphate can be regarded as a native reporter group of phosp...

ORDERED MECHANISM OF BINDING OF PHOSPHORYLASE KINASE AND GLYCOGEN PHOSPHORYLASE b TO GLYCOGEN

The kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen has been studied using turbidimetric method (40 mM Hepes, pH 6.8, and 8.2; 20◦C). The binding of phosphorylase kinase with glycogen occurs only in the presence of Ca and Mg . According to the kinetic data, phosphorylase b favors the binding of phosphorylase kinase with glycogen. This conclusion is suppo...

Oligosaccharide binding to glycogen phosphorylase b.

Synthesis of heterocyclic N-(b-D-gluco of glycogen phosphorylase

In a DCC-mediated cou N-propynoyl-2,3,4,6-tet cycloadditions with aro copyranosyl)-1-substitu isoxazole-5-carboxamid to be tested as inhibitor were N-(b-D-glucopyra N-(b-D-glucopyranosyl)1

Molecular studies of glycogen phosphorylase b from bovine liver.

Two active isoforms of bovine liver phosphorylase with distinct subunit composition have previously been purified (Cámara Artigas, A., Barón, C. and Parody-Morreale, A. Prot. Express. Purif. 1994, 5, 157), one showing three SDS-PAGE polypeptide bands (molecular mass = 97, 55 and 40 kDa) and the other showing just one (molecular mass = 97 kDa). A molecular mass of 200 kDa has been determined for...